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ISOLATION AND IDENTIFICATION OF Staphylococci FROM BODY SURFACE OF HUMANS AND THEIR SENSITIVITY TO COMMONLY USED ANTIBIOTICSBala AuwaluABSTRACT
Isolation and identification of Staphylococci was intended to isolate Staphylococcus species from body surfaces and determine their sensitivity to the commonly used antibiotics. A total of seventy three (73) skin swab samples were collected from student’s resident at the hostels of Usmanu Danfodiyo University, Sokoto, Nigeria. Using bacteriological and biochemical identification methods, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus warneri and streptococcus species were isolated. Out of the isolates obtained on manitol salt agar, 29 (30.21%) were identified as S. aureus, 44 (45.83%) were identified as S. epidermidis, 10 (10.42%) were identified as S. warneri, 13 (13.54) were identified as Streptococcus species. Antibiotics used for sensitivity test Amoxyclav, Cefalaxin, Ciprofloxacin, Clindamycin, Co-trimoxazole, Erythromycin and Tetracycline using disc diffusion method, reveal that S. aureus had the highest resistance (84.48%), followed by S. warneri (20.00%) and then S. epidermidis (15.34%). S. aureus have now continued to become resistant to most of the common antibiotics used locally.
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